Home|Journals|Articles by Year

Directory for Medical Articles
 

Original Research

Egypt. J. Exp. Biol. (Zoo.). 2012; 8(2): 273-285


HISTOLOGICAL, HISTOCHEMICAL, AND IMMUNOHISTOCHEMICAL STUDIES OF THE CARDIAC MUSCLE OF THE ALBINO RAT UNDER IMMOBILIZATION STRESS AND THE CURATIVE ROLE OF DIAZEPAM

Nabila I. El-Desouki Amal I. El-Refaiy Dalia F. A. Abou-Zaid Amira A. Abdel - Kader.


Abstract

The present study is planned to study the effect of immobilization stress on the cardiac muscle of adult male albino rats and the curative role of diazepam. The study was carried out on 80 albino rats; the animals were divided into eight groups: group 1 served as control rats; group 2 unstressed rats injected intraperitoneally daily with 0.1 mg/kg b.w. diazepam for 30 days; groups 3, 4, and 5 served as immobilized stressed-rats for 30 days (by restricting movement for 2 hr daily at different durations 5, 15, and 30 days, respectively); groups 6, 7, and 8 stressed rats treated daily with 0.1 mg/kg b.w. diazepam for 30 days. The histological study of the cardiac myofibres of the stressed-rats revealed disorganization of the muscle fibres, vacuolation of the sarcoplasm, pyknosis of the nuclei, congestion and dilatation of blood vessels in endomysium. The thickness of collagen fibres gradually increased and became compact dense in the stressed rats till 30 days, and they were more obvious around the blood vessels. The histochemical study demonstrated a marked reduction in the glycogen and protein contents of the cardiac muscle, and was time-dependent. The immunohistochemical study revealed that the rats stressed for different durations manifested the disappearance of the cytoskeletal desmin protein filaments at intercalated discs and Z-lines of the cardiac myofibres. Treatment with diazepam for 30 days to stressed rats demonstrated markedly improvements of the architecture of cardiac myofibres and restoration of the two main chemical components; glycogen and protein contents. Also, restoration of desmin in the cardiac myofibres was elucidated. The results indicated that diazepam is recommended to be used as a curative drug to improve the disturbances in the cardiomyocytes and myofibrils assembly caused under the effect of stress.

Key words: Cardiac muscle, Desmin, Diazepam, Histochemistry, Histology, Immunohistochemistry, Rat, Stress






Full-text options


Share this Article



Online Article Submission
• ejmanager.com
• ojshosting.net







Do you want to use OJS for your journal ?
work with an experienced partner
www.OJSHosting.net

eJManager.com
Review(er)s Central
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.