Acetic Acid Bacteria (AAB) are Gram-negative bacteria, live under strict need of oxygen. They are characterized by their ability to undergo incomplete alcohol and sugar oxidations to produce organic acids as end products. They are ubiquitous in niches with high sugar concentrations and most of them have potent capabilities to oxidize ethanol to acetic acid. These criteria make AAB very good candidates to be exploited in different industrial applications. AAB taxonomy subjected to many changes making it very attractive for further investigations. Our work aims to identify some isolates belonging to family Acetobacteraceae using 16sr-DNA molecular typing technique. After isolation, 48 isolates were identified as members of Acetobacteraceae. Four out of them identified as Gluconoacetobacter sacchari, Acetobacter pasteurianus, Gluconobacter fraturii and Acetobacter malorum by 16sr-DNA molecular typing technique. Neighbor-joining phylogenetic tree based on 16s-rDNA sequences analysis for the four identified isolates was created showing the relationship among these isolates and representative species of the genera Acetobacter, Gluconoacetobacter and Gluconobacter.
Key words: Acetobacter, Gluconobacter, Gluconoacetobacter, Sequencing, 16sr-DNA.
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