Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles
 

Open Access

Original Research

Egypt. J. Exp. Biol. (Bot.). 2011; 7(2): 153-162


CHARACTERIZATION OF A NEW LONG ACTIVE STRAIN OF LETTUCE MOSAIC VIRUS INFECTING LACTUCA SATIVA IN EGYTPT

Ismail M. Al-Sharkawi.

Abstract
Characterization of a new resistant strain of lettuce mosaic virus was carried out. The results showed that the virus was sap transmissible and also transmitted by the seeds. The virus thermal inactivation point is recorded as 100oC for 30 minutes, dilution endpoint was 1:8x10-6 and the virus still active for 210 days at 20-30oC. Electron microscopic examination of the virus revealed the presence of flexuous rod-shaped virus particles with mean length of 13x740nm. The virus had a wide host range of which showed varied forms of local lesions. Few hosts show local lesions followed by systemic symptoms. The histopathological studies of Hibiscus schizopetalus leaves infected with the virus and showing necrotic local lesions revealed that the blade is not clearly flat. The thickness of the blade is unequal on both sides of the midrib. The upper and lower edges of the midrib showed clear collenchyma cells. The epidermal cells have few distorted stomata and are covered with thick cuticle. The mesophyll cells appear compact without differentiation into palisade and spongy tissues. The chloroplasts are few and the numbers of druses calcium oxalate crystals are clearly few. Necrotic spots appear on both upper and lower epidermis densely stained with safranin. The virus has no effect on xylem and phloem cells. The parenchyma cells surrounding the vascular tissue appear wavy walled.

Key words: Lettuce mosaic virus, Lactuca sativa, Thermal inactivation point, Histopathology, Local lesions, Symptoms, Host range



Share this Article


Advertisement
American Journal of Diagnostic Imaging

SUBMIT YOUR ARTICLE NOW


ScopeMed.com
BiblioMed Home
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.