An efficient plant regeneration protocol through two-stage culture of nodal segment has been reported here for a valuable medicinal plant, Bacopa monnieri. Multiple shoots with large number of shoot buds were induced from nodal explant on Murashige and Skoogs (1962) (MS) medium fortified with (1.0-5.0 mg L-1) N6- benzyladenine (BA). Of the different concentrations of BA tested, 3.0 mg L-1 BA offered the best result, where the nodes swelled and an average of 6.5 shoots with numerous buds/nodes were recorded. The swollen nodes, cultured on MS + 3.0 mg L-1 BA, were sub-cultured either on MS or MS supplemented with 1.0 mg L-1 GA3 medium. The best result (114.2 shoots/ node) with an average shoot length of 6.4 cm was observed on MS media supplemented with 1.0 mg L-1 GA3. Cent per cent acclimatization of healthy in vitro regenerated shoots was obtained by ex vitro rooting in plastic pots containing garden soil, which saves time and tissue culture cost by abolishing the in vitro rooting step.
Key words: Axillary shoot proliferation, Bacopa monnieri, Medicinal plant, Two-stage culture, Ex vitro rooting
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