Abstract

Biomolluscicides development is of great interest in pest management to avoid the effects on the environment caused by synthetic molluscicides. The Species: Solanum mammosum L., Sapindus saponaria L. and Jatropha curcas L. have demonstrated molluscicidal properties against Pomacea canaliculata. This study was carried with mixtures of aqueous extracts of these plants in order to check for a synergistic effect between them. Extracts obtained from each species were evaporated and lyophilized. By using a simplex centroid design were obtained ten mixes for testing. All of them were diluted to 500ppm and tested on adult snails for 24 hours of exposure followed by 24 hours of recovery on tab water. Those mixes that showed the best results of mortality were chosen to determine the median lethal dose. The formulations S. saponaria (100%) and S. mammosum - S. saponaria (50%-50%) showed the best results of mortality with LD50 values of 24.04 ppm and 17.78 ppm respectively, with no significant differences between them (p> 0.05). These results showed that a synergistic effect is not produced in the activity of these plants when the extracts mixture was performed.

Key words: Bioassay, molluscicide, Pomacea canaliculata