Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles

Open Access

Regular Article

Emir. J. Food Agric. 2012; 24(5): 434-443

Influence of inflorescence explant age and 2,4-D incubation period on
somatic embryogenesis of date palm

Adel Ahmed Abul-Soad*.

The objective of this study was to determine the appropriate growth stage of inflorescence (spathe) explantsamong different cultivars of the date palm (Phoenix dactylifera L.) and to define the optimum period ofincubation on high-auxin medium. Inflorescence explants were excised from seven Pakistani cvs. at differentgrowth stages and cultured on a basal MS medium containing 100 mg l-1 2,4-D for 3, 6 and 24 weeks (BatchesI, II and III) before being transferred to 10 mg l-1 2,4-D medium. The cultures were incubated at 25 2C in thedark and transferred onto fresh culture medium every 3 weeks. Results revealed that spikelet explants isolatedfrom different cultivars with the same length were not necessarily at the same stage of development. Lengthvaried according to cultivar, time of excision, location of the inflorescence in the crown and location of thespikelets in the inflorescence. Accordingly, the response differed among the cultivars tested. After 24 weeksfrom initial culture, some Batch II explants produced only unfriable callus and embryos. On the other hand,explants comprising Batches I and III failed to induce organs even after 42 weeks (14 subcultures); instead theyturned blackish brown. Multiple somatic embryos were subjected to proliferation at the multiplication stage.Individual shoots were rooted and successfully transplanted in the greenhouse with about 90% survival.

Key words: Date palm, Inflorescence, Micropropagation, Embryogenesis, Phoenix dactylifera

Share this Article

Journal of Molecular Pathophysiology


BiblioMed Home
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.