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Application of response surface methodology (RSM) in condition
optimization for essential oil production from Citrus latifolia

Quoc Le Pham Tan*, Xinh Nguyen Thi Kieu, Nguyet Huynh Thi Kim and Xuyen Nguyen Thi Hong.

Abstract
This research shows the dependence of distillation time and ratio of water/peel on amount of essential oil afterdistilling. Researching and optimizing the factors for essential oil production by laboratory ? scale distillationand the yield was evaluated using the Response Surface Methodology (RSM) with Central Composite FaceCentered(CCF) model. The regression equation obtained was shown below: 2 212 1 2 y = 0.997 - 0.015x + 0.04x - 0.107x - 0.042x .There is the contrast between distillation time and ratio of water/peel with volume of essential oil received.Optimal result is volume of essential oil was 1 ml (y) with quantity of lemon peel/batch of 50g, ratio ofwater/peel (x1) was 6/1, distillation time (x2) in 20 minutes at temperature at 100oC. The essential oil from thepeel of C. latifolia was analysed by GC-MS method. A lot of compounds were identified of which limonene, ??pinene and ??terpinenen were major components. Small amounts of trans-caryophyllene, 4-terpineol, ?-terpinenen?were also detected.

Key words: CCF, C. latifolia, Essential oil, GC-MS, RSM



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