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In vitro production of Stevia rebaudiana Bertoni

M. Anbazhagan, M. Kalpana, R. Rajendran, V. Natarajan, D. Dhanave.

Cited by (7)

Abstract
Experiments were conducted for the standardization of in vitro culture technique for themass propagation of Stevia rebaudiana, a medicinally important, zero-calorie value, sweet tastedand an antidiabetic herb. Shoot tip, nodal segment and leaf were used as explants and they werecultured on Murashige and Skoog (MS) medium supplemented with different concentrations ofBA, Kn and IAA both in individual and in combined form for shoot inductions and the best resultswere obtained from MS medium supplemented with BA+ IAA at the concentrations of 1.0 mg/land 0.5 mg/l respectively. Among the explants used, shoot tip stood first in inducing shootdevelopment. Best root formation of in vitro developed shoots could be achieved on half-strengthNitsch (N6) medium supplemented with IAA at concentration 1.0 mg/l. The in vitro developedplantlets were transferred to pot and they were grown in greenhouse for hardening and finally theywere planted in the open filed. Around 82% of plants were successfully established in natural fieldcondition.

Key words: in-vitro, Stevia rebaudiana, zero-calorie, MS and N6 medium.



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