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Evaluation of genetic variation in mutants of Black gram (Vigna mungo L.) Hepper) as revealed by RAPD markers

D. Arulbalachandran1, L. Mullainathan1, S. Karthigayan, S.T. Somasundaram2 and S. Velu1.

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One of the most possibilities is the genetic marker system to find out the geneticvariation. The advent of large-scale DNA sequencing technology has generated a tremendousamount of sequence information for many important organisms. The genetic variation wasevaluated in four mutants (high seed protein, tall, bushy and dwarf mutants) along with parentcultivar (control) by 20 random primers which generated 202 fragments scored with 58polymorphic DNA bands. The average DNA bands were 10.1 per locus ranged from 1 to 9. Theaverage polymorphic rates were 38.37 % among mutants and parent cultivar (control) through the20 primers. Primer OPK–06 and OPK-11 revealed 62.5 % DNA polymorphism. Five genotypeswere used to constructed dendrogram based on the similarity matrix, suggested that geneticdistance from 0.621 to 0.785. The DNA variation might have been caused by mutation due togamma rays and ethyl methane sulphonate. Hence, research is needed to analyse function ofmutated genes for their mutants characters for future prospects because, such mutants and theirgenes when using in cross breeding/transgenic technologies will found more production in thedevelopment of improved crop varieties like high seed protein, lodging resistances, semi dwarfwith high yield.

Key words: Gamma rays, Ethyl methane sulphonate, DNA polymorphism.

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