Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles

Open Access

Regular Article

Influence of equilibration time during vitrification and culture medium on development of in vitro-derived bovine embryos

Abdulwali M. Al-aghbari,
Alfred R. Menino Jr..

The objective of the present study was to examine the influence of culture mediumand equilibration time during vitrification on development of cryopreserved in vitro-derivedbovine embryos. Following insemination, 50% cumulus-enclosed presumptive zygotes weredesignated as controls and returned to the original microdrops for further incubation and theremaining of the embryos microdrops, randomly assigned into two culture groups, TCM-199 containing 10% (v/v) fetal bovine serum and SOM prepared with the elevated potassium formulation (KSOM) supplemented with 1%BSA were vitrified. Embryos were recovered from the cryo-vials and warmed at 37oC in 0.3 M trehalose in TCM-199 for 1-2 min. Each group of embryos was immediately washed 3 times with its respective culture medium, KSOM or TCM, and returned to their corresponding microdrops containing the cumulus cell complement. Embryos were cultured for an additional seven days and evaluated at 24-h intervals to assess development. Development of presumptive bovine zygotes was similar in KSOM and TCM prior to vitrification. Development of bovine embryos was significantly greater (P0.10) percentages to control and both were greater (P

Key words: Keyword

Share this Article

American Journal of Research in Medical Sciences


BiblioMed Home
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.