Home|Journals|Articles by Year|Audio Abstracts

Regular Article

Leaf-derived organogenesis in vitro for mass propagation of lisianthus (Eustoma grandiflorum (Raf.) Shinn

Budi Winarto, Fitri Rachmawati, Anggraeni Santi Setyawati and Jaime A. Teixeira da Silva.


Lisianthus (Eustoma grandiflorum (Raf.) Shinn) is an important ornamental commodity in South-East Asia. However, mass propagation of the plant at a commercial scale to satisfy market demands is faced by limited availability of high quality and uniform seedlings as planting material. Using different regeneration media and leaf explants for callus induction, regeneration, proliferation, root formation and acclimatization were studied. High callus induction and adventitious shoot formation were possible from leaf explants of E. grandiflorum ‘White Lavender’ cultured on Murashige and Skoog (MS) medium supplemented with 3.0 mg/l thidiazuron (TDZ) and 0.3 mg/l α-naphthalene acetic acid (NAA), but high quality shoots (8.0) was established on MS medium containing 0.5 mg/l N6-benzyladenine (BA) and 0.002 mg/l NAA. In the same medium, adventitious shoots could be multiplied up to the fourth subculture at a rate of 1.74 which decreased to a 1.57 multiplication rate in subsequent subcultures. Shoots rooted easily on MS medium containing 0.1 mg/l BA and 0.02 mg/l NAA with 3.9 roots per shoot. The plantlets, which were successfully acclimatized in a mixture of burned-rice husk and organic manure (1:1, v/v) with 90% survival, grew well after repotting.

Key words: Adventitious shoots, Eustoma grandiflorum, leaf explant, regeneration medium, tissue culture

Full-text options

Share this Article

Online Article Submission
• ejmanager.com

ejPort - eJManager.com
Review(er)s Central
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under Creative Commons Attribution 4.0 International License (CC BY), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.