Home|Journals|Articles by Year

Directory for Medical Articles
 

Regular Article



In vitro pseudobulb based micropropagation for mass development of Cymbidium finlaysonianum Lindl.

Shahinul SM Islam, Touhidul Islam, Bakul Bhattacharjee, Tushar Kanti Mondal, Sreeramanan Subramaniam.


Abstract

An efficient protocol for micropropagation using the axenic pseudobulb derived from in vitro germinated seedlings of Cymbidium finlaysonianum Lindl was developed. The immature seeds were germinated on MS0 basal medium for seedling development. In vitro grown seedlings responded well and stimulated pseudobulb formation with benzylamino purine (BAP) 1.0 mg/L + α-naphthalene acetic acid (NAA) 0.5 mg/L on MS medium. For multiple shoot induction, the axenic pseudobulb segments excised from 8 month old seedlings were cultured on the medium containing different concentrations and combinations of growth regulators (NAA, BAP and Kinetin). Best response for shoot multiplication and elongation was obtained in medium with 0.75 mg/L NAA and 1.5 mg/L BAP. The individual shoots were transferred to half-strength MS medium supplemented with 0.5 to 2.0 mg/L of indole-3 acetic acid (IAA), indole-3 butyric acid (IBA) and naphthalene acetic acid (NAA) to observe root development. Half strength of MS + 1.00 mg/ L IAA has proven the best for root induction. The well rooted plants were hardened successfully in the potting mixture containing coconut husk, charcoal pieces (5-7 mm size), decaying litter and very small brick pieces (5-7 mm size) in the ratio of 1:1:1:1 and eventually plants are established under natural condition. The present investigation can be used for rapid mass propagation of this highly important Cymbidium finlaysonianum orchid species.

Key words: Axenic pseudobulb, Cymbidium finlaysonianum, Micropropagation, Multiple shoot, Plant growth regulators






Full-text options


Share this Article



Online Article Submission
• ejmanager.com







eJManager.com
Review(er)s Central
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.