Aim: This study evaluated the antimicrobial and antioxidant effects of Crinum jagus (J. Thomps.) Dandy methanolic bulb extract in wound healing. Methods: Phytochemical screening revealed the presence of alkaloids, glycosides, tannins and saponins in the extract. In vitro antimicrobial activity of the extract was determined by agar well diffusion method. In vivo antimicrobial activity of the extract was determined by assay of excision wound in rats contaminated with Staphylococcus (S.) aureus, Bacillus (B.) subtilis, Pseudomonas (P.) areuginosa and Candida (C.) albicans and treated with 300mg/kg body weight (bw) of 10 and 5% methanolic Crinum jagus bulb extract ointment (MCJBEO), respectively. Enzymatic antioxidant effect of the extract was determined by assaying superoxide dismutase (SOD) and catalase (CAT) activity, and malondialdehyde (MDA) level in excision wound biopsies of rats treated with 10 and 5% MCJBEO, respectively. Non-enzymatic antioxidant effect of the extract was determined in vitro using diphenylpicrylhydrazyl (DPPH) method. Results: The extract exhibited in vitro antimicrobial effect in a concentration-dependent manner with 100mg/ml having the highest inhibitory zone diameter (IZD) for B. subtilis (25mm), S. aureus (21mm) and C. albicans (14mm). B. subtilis, S. aureus and C. albicans were not isolated from wounds of animals treated with both extract concentrations, and reference drug (framycetin sulphate/clotrimazole). SOD and CAT in wound biopsies treated with 10% MCJBEO were significantly (p < 0.05) higher than those treated with 5% MCJBEO. Significantly (p < 0.05) decreased MDA level of wound biopsies from extract-treated rats was observed. The extract exhibited non-enzymatic antioxidant (DPPH) effect in a concentration-dependent manner. Conclusion: Antimicrobial and antioxidant effects could possibly be part of mechanism by which Crinum jagus bulb extract promote wound healing process.
Key words: Crinum jagus, in vivo, antimicrobial, antioxidant, wound healing
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