Abstract

In the present research, the reverse phase (RP)-high performance liquid chromatography (HPLC) method was developed and validated to determine phytochemical markers andrographolide, eugenol, and zingerone in nilaveembu kudineer (NK) a Siddha polyherbal formulation. There are no RP-HPLC methods reported for the simultaneous estimation of these marker compounds in any herbal formulation. In this study, the optimized chromatographic conditions were C18 Column (150 × 4.6 × 5 μm) mobile phase containing Acetonitrile and 0.1% Ortho Phosphoric acid 30:70, flow rate 1.0 ml/minutes, at 254 nm using a ultravoilet detector, column temperature of 40°C and run time of 20 minutes. In the linearity study, a linear relation was observed from 25 to 200 μg/ml with a correlation coefficient of 0.9990. The optimized method is successfully applied for the estimation of these phytochemical markers in NK formulation. The suggested approach is an excellent quality control tool for the simultaneous quantitative assessment and detection of phytochemical markers present in polyherbal formulations and in traditional medicines such as ayurveda, siddha, unani, and homeopathic formulations.

Key words: Andrographolide, Eugenol, Nilaveembu Kudineer, Phytomarkers, RP-HPLC, Zingerone,.