The present study was designed to determine the phytocomponents of the ethanol extract of N. nucifera seed using GC-MS. The phytochemical screening of the ethanolic extract of N. nucifera seed was performed using Perkin-Elmer Gas Chromatography - Mass Spectroscopy. GC-MS chromatogram of the ethanolic extract of N. nucifera seed showed thirty-eight peaks which indicating the presence of thirty-eight phytochemical constituents. The major phytochemical constituents are lupeol (22.95%), octadecatrienoic acid-2-[(trimethylsilyl)oxy]-1-[[(trimethylsilyl)oxy] methyl]ethyl ester (Z,Z,Z) (7.86%), 2,3-Dihydroxypropyl-cis-13-docosenoate (5.35%), oleanolic acid(5.19%), tristrimethylsilyl ether derivative of 1,25-dihydroxyvitamin D2 (3.36%) ,trimethylsilyl derivative of 2-monoolein (3.26%), lucenin 2 (3.02%), Lupanol (2.95%), Tetraneurin-A-diol (2.78%),Carotene-1,1',2,2'-tetrahydro-1,1'-dimethoxy (2.32%),1-Oxo-forskolin (1.99%), oleic acid-3-hydroxypropyl ester (1.78%), oleic acid trimethylsilyl ester (1.69%), Betulin (1.61%), à-Amyrin-trimethylsilyl ether (1.49%),Stigmast-5-en-3-ol-(3á,24S)(1.43%), Flavone-4'-OH,5-OH,7-di-O-glucoside (1.11%). Among thirty-eight compounds identified, only twelve were reported to have biological activities. Our results indicate that N. nucifera seed contain various bioactive components.
Key words: Nelumbo nucifera, phytocomponents, ethanolic extract, GC-MS analysis