Home|Journals|Articles by Year Follow on Twitter

Directory for Medical Articles
 

Open Access

Original Article

J App Pharm Sci. 2015; 5(3): 073-079


HPTLC finger printing profile and evaluation of in vitro antidiabetic potential of medicinally important plant Cassia obtusa L. (Caesalpiniaceae)

K. Thenmozhi, S. Jamuna, K. Karthika, S. Manian, S. Paulsamy, C. Chitravadivu.

Abstract
Objective: To establish the finger printing profile and evaluation of in vitro antidiabetic potential of C. obtusa. Methods: In vitro antidiabetic activity was carried out according to the method adopted by Miller, 1959. HPTLC studies were carried out using CAMAG HPTLC system equipped with Linomat 5 applicator, TLC scanner 3 and Win cats-4 software for the active fractionation of aqueous-methanolic leaf extracts of C. obtusa. Results: Among the various plant parts analyzed, leaf exhibited efficient inhibitory activity against α-amylase and α-glucosidase enzymes. Therefore, the leaf extract was further fractionated using various solvent systems petroleum ether, chloroform, ethylacetate, butanol and water and were subjected to in vitro antidiabetic activity. Among the fractions analyzed chloroform fraction exhibits remarkable antidiabetic activity by inhibiting α-amylase and α-glucosidase enzymes. Furthermore, the active leaf fractions were analyzed with HPTLC to develop fingerprint profiles and these fractions exhibited the presence of 13 and 22 major spots of alkaloids and flavonoids respectively with different Rf values. Conclusion: The results of the present study thus claim the folkloric usage of the plant in diabetic related maladies.

Key words: Cassia obtusa, antidiabetic activity, HPTLC fingerprint profile, alkaloids, flavonoids.



Similar Articles

Study on the antioxidative mechanism of tocopherol loaded ethyl cellulose particles in thermal-oxidized soybean oil.
Liu Y, Ma X, Li J, Fan L, Huang S
Carbohydrate polymers. 2022; 276(): 118734

Improving cell transplantation by understanding and manipulating the phagocytic activity of peripheral glia.
Nazareth L, St John J, Ekberg J
Neural regeneration research. 2022; 17(2): 313-314

Effect of cellulose nanocrystal-stabilized cinnamon essential oil Pickering emulsions on structure and properties of chitosan composite films.
Liu J, Song F, Chen R, Deng G, Chao Y, Yang Z, Wu H, Bai M, Zhang P, Hu Y
Carbohydrate polymers. 2022; 275(): 118704

Iminoboronate-chitooligosaccharides hydrogels with strong antimicrobial activity for biomedical applications.
Ailincai D, Rosca I, Morariu S, Mititelu-Tartau L, Marin L
Carbohydrate polymers. 2022; 276(): 118727

Structural analysis and anti-cancer activity of low-molecular-weight chondroitin sulfate from hybrid sturgeon cartilage.
Wu R, Li P, Wang Y, Su N, Xiao M, Li X, Shang N
Carbohydrate polymers. 2022; 275(): 118700


Full-text options


Latest Statistics about COVID-19
• pubstat.org


Add your Article(s) to Indexes
• citeindex.org






Covid-19 Trends and Statistics
ScopeMed.com
CiteIndex.org
CancerLine
FoodsLine
PhytoMedline
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.



ScopeMed Web Sites