Lactobacillus acidophilus DSMZ20079T was used in this study to effectuate selenium nanoparticles (SeNPs) by transforming toxic soluble selenite oxyanions (SeO32-) into red insoluble elemental selenium (Se0). The strain was able to withstand, tolerate, and grow in the presence of high levels of selenite toxicity at concentrations ranging from 1 mM to 10 mM. Produced SeNPs were purified and subsequently characterized systematically using UV–Vis spectroscopy, Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), Energy dispersive X-ray spectroscopy (EDX), Fourier Transform Infrared (FTIR) techniques. The absorbance scan of UV–Vis showed a broad plasmon peak at 260 nm. The TEM microscopy showed spherical-shaped Se0 nanoparticles with a size ranging from 8 to 23 nm. XRD results revealed crystalline shape with a mean size of 13.23 nm. This research focuses on the synthesis of selenium nanoparticles from Lactobacillus acidophilus DSMZ20079T which has the potency to function as a natural manufactory.
Key words: Selenium, Lactobacillus acidophilus, biosynthesis, sodium selenite, nanoparticles, characterization.
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