The present paper aims to develop a simple direct colorimetric method for the determination of cerberin in rat plasma without any previous chemical separation of the cerbrin from Cerbera odollam. The method is based on reaction of the cardenolide group of cerberin with 3,5-dinitro salicylic acid [DNS] in alkaline medium which yields a bright orange-yellow complex that exhibits absorption maxima at 370 nm. Beers law obeyed in the concentration range of 50-250 µg/ml. The result of the method was validated statistically and by recovery studies.
Key words: Cerberin, biological fluid ,visible spectrometry .
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