Objective: The aim of this study was to compare the disinfection efficacy of various disinfectants on gutta-percha root canal filling materials.
Methods: Three-hundred and seventy standard #80 gutta-percha cones were used for the study. The gutta-percha cones were sterilized with ethylene oxide. Ten cones were separated for negative control group. Then the gutta-percha cones were randomly divided into four groups containing 90 points each and contaminated with Escherichia coli, Enterococcus faecalis, Candida albicans and Streptococcus mutans. Specimens were incubated in 10 ml Brain Heart Infusion Broth at 37°C for 72 h. After incubation, cones were dried with sterile gauze; ten of the cones from each group were seperated for positive control groups and rest of the cones were divided into four subgroups according to the chemical disinfectant used (n = 80 each): 5.25% sodium hypochlorite (NaOCl); 2% chlorhexidine gluconate (CHX); 10% povidone iodine (PI); and MTAD groups. For each subgroup, the gutta-percha cones were immersed into the disinfectant for 1 and 10 min individually. In plates showing growth, the number of colony forming units were counted.
Results: NaOCl, CHX and MTAD were equally effective on tested microorganisms.
Conclusion: 5.25% NaOCl, 2% CHX, and MTAD are effective agents for a rapid high disinfection level of gutta-percha cones.
Antimicrobial activity, disinfection, endodontics, gutta-percha cones, MTAD