Aim: The aim of the present study was to investigate the antioxidant and hepatoprotective effects of polyherbal formulation (PHF) containing Cajanus cajan (L.) Millsp., Lawsonia inermis L. Linn, Mimosa pudica L., Uraria picta (Jacq.) DC. and Operculina turpethum (L.) Silva Manso on carbon tetrachloride (CCl4) induced acute liver damage in albino rats.
Methods: The groups of animals were administered with PHF at the doses 100, 200 and 400mg/kg b.w. (p.o.) once in a day for 7 days and at day 6th and 7th the animals were administrated with Carbon tertrachloride (1.0 mL/kg b. w. 50% v/v with olive oil,; p.o.). The effect of PHF on SGPT (Serum glutamine pyruvate transaminase), SGOT (Serum Glutamine oxaloacetate transaminase), alkaline phosphatase (ALP) and total bilirubin were determined in CCl4induced hepatotoxicity in rats. Further, the effects of PHF on glutathione (GSH), Superoxide dismutase (SOD) level and lipid peroxidation (LPO) activity were also investigated.
Results: The results demonstrated that PHF (400 mg/kg b.w.) significantly reduces the CCl4 induced increase in level of serum SGPT, serum ALP and total bilirubin. PHF (400 mg/kg b.w.) prevents the depletion level of GSH and decrease in the activity of SOD in CCl4 induced liver injury in rats. In addition, PHF also showed a significant decrease in the LPO levels signifying the potent antioxidant activity.
Conclusion: All of our findings suggest that PHF could protect the liver cells from CCl4induced liver damages and the mechanism may be through the antioxidative effect of PHF.
Key words: Hepatoprotective, Antioxidant, Polyherbal Formulation, Carbon Tetrachloride
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