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Original Article



Impact of chilling duration on sperm quality of indigenous buck semen in the Coastal Area of Bangladesh

M. Swarna, S. Biswas, D. Biswas, N. G. Saha, A. K. Paul.




Abstract

Background: Assessing semen characteristics is an important step before semen preservation. This study aimed to collect, evaluate, and preserve indigenous buck semen in the coastal area of Bangladesh.
Methods: The study was conducted at the Theriogenology and Animal Reproductive Biotechnology Laboratory, Department of Medicine, Surgery and Obstetrics, Patuakhali Science and Technology University, from July 2021 to June 2022. We selected four bucks based on non-return rate, pregnancy rate of does, and various parameters including age, body weight, scrotal circumference, and testicular epididymal length. These selected animals received a regimen of vitamins, minerals, anthelmintics, and concentrated feed. Semen was collected using the artificial vagina method, and data on color, odor, volume, viscosity, mass activity, consistency, concentration, and individual sperm motility were recorded and analyzed after collection.
Results: The semen of bucks exhibited a creamy white color and a fishy odor. The mean (±SD) of scrotal circumference (SC) of four bucks was 20.43±0.52, while the testicular epididymal length (TEL) was 11.03±0.59. Buck-2 (B-2) and buck-1 (B-1) showed significantly (p

Key words: Artificial vagina, scrotal circumference, mass activity, motility






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