An attempt was made to isolate and screen efficient amylolytic strains of Bacillus sp. Initial screening based on Starch hydrolysis ratio (SHR) resulted in selection of 72 amylolytic bacterial strains. Among these, 18 strains were selected for further studies. Secondary screening based on amylase production in starch broth medium led to selection of six amylolytic strains of Bacillus sp. The selected strains were grown in four different fermentation media (FMI-FMIV) in order to screen for three most efficient amyloytic strains for optimization and characterization. FMIV was the best basal medium for amylase production as it had the shortest incubation time (24 hours) and highest amylase production. Molecular identification based on 16S rDNA sequence revealed that three most efficient strains (BCM36 (KR1), BCM33 (KR2) & BCM25 (KR3)) belonged to Bacillus sp.
Key words: Amylase, fermentation medium, 16S rDNA sequence,Bacillus sp.
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