Aim: This study assessed within 4 days of suppressive test in vivo antimalarial activity of Ethanolic extract of root and stem bark of Cassia sieberiana DC against chloroquine sensitive strain of Plasmodium berghei berghei NK65 in mice.
Methods: Two sets, each of five groups of four mice per each group were used. The groups of animals were administered with 100, 200, and 300 mg extract/kg body weight respectively, while positive control group were administered with 5 mg chloroquine/kg body weight and the negative control, were administered with 5m1 distilled water/kg body weight. Oral acute toxicity was evaluated using up and down procedure.
Result: Both the root and stem bark extract of C. sieberiana showed antimalarial property for suppressive tests. Chemo suppression of the root extract exerted significant (P < 0.05) dose dependent reduction in level of parasiteamia of 30.7%, 52.7% and 55.8%. And from stem extract 17.6%, 38.0%, and 63.9%.were recorded on mice as compared to 96.0% suppressive rate obtained from weight of chloroquine. The phytochemical screening of the plants root and stem bark extract revealed the presence of alkaloids, anthraquinones, flavonoids, triterpenoids, tannins, cardiac glycosides, saponins, reducing sugars, and carbohydrates. The oral median lethal dose was determined to be >3000 mg/kg body weight.
Conclusion: The acute toxicity results of this study showed that the plant parts used are assumed to be safe and has antiplasmodial activity which can be explored for the management of malaria.
Key words: antimalaria, phytochemicals, suppressive test
|
