Abstract

Objectives: This study aimed at investigating the genotoxicity and cytotoxicity effect of Tapinathus globiferus and Zanthoxylum zanthoxyloides to human leukocytes. In addition, the reductive potential and the chemical composition of the two plant extracts were also determined. Methods: Human leukocytes were obtained from healthy volunteer donors. The genotoxicity and cytotoxicity of T. globiferus and Z. zanthoxyloides were assessed using the Comet assay and Trypan blue exclusion respectively. The antioxidant activity of the plant extracts was evaluated by the reducing power assay. Furthermore, HPLC-DAD was used to characterize and quantify the constituents of these plants. Results: T. globiferus (10-150 µg/mL) was neither genotoxic nor cytotoxic at the concentrations tested, suggesting that it can be consumed safely at relatively high concentrations. However, Z. zanthoxyloides showed cytoxicity and genotoxicity to human leukocytes at the highest concentration tested (150 µg/mL). In addition, the total reducing power of T. globiferus was found higher than Z. zanthoxyloides in potassium ferricyanide reduction. Both plants extract contained flavonoids (rutin and quercetin) and phenolic acids (chlorogenic and caffeic). Conclusion: The results obtained support the fact that some caution should be paid regarding the dosage and the frequency of use of Z. zanthoxyloides extract.

Key words: Tapinathus globiferus; Zanthoxylum zanthoxyloides; cytoxicity; genotoxicity; HPLC-DAD