Home|Journals|Articles by Year Follow on Twitter

Directory for Medical Articles
 

Open Access

Original Research



Effect of ERN1 knockdown on the expression of MAP3K5, MAP4K3, CIB1, RIPK1, and RIPK2 genes in U87 glioma cells and its hypoxic regulation

Dmytro O Minchenko, Taia V Bakalets, Daria O Tzymbal, Oksana O Ratushna, Yulia M Bashta, Olelsandr O Minchenko.

Abstract
ERN1 (endoplasmic reticulum to nucleus signaling 1)-mediated signaling pathway plays an important role in glioma growth. We studied the possible role of protein kinases MAP4K3, MAP3K5, RIPK1, and RIPK2 as well as protein kinase interacting protein CIB1 in suppression of U87 glioma cell proliferation upon ERN1 knockdown. It was shown that blockade of ERN1 signaling enzyme function significantly decreases the expression level of genes encoding MAP4K3, RIPK2, and CIB1 in U87 glioma cells, but increases expression of RIPK1 gene. At the same time, the blockade of ERN1 signaling enzyme does not affect the expression of MAP3K5 gene in glioma cells. Hypoxia down-regulates the expression level of MAP3K5, MAP4K3, RIPK1, and RIPK2 mRNAs in control glioma cells. However, inhibition of ERN1 affected hypoxia-mediated changes in expression of these kinase genes and their magnitude. Results of this investigation clearly demonstrated that the expression of MAP4K3, RIPK1, RIPK2, and CIB1genes in U87 glioma cells in human U87 glioma cells relies on blockade of ERN1-mediated endoplasmic reticulum stress signaling and is mostly down-regulated by hypoxia in dependence of ERN1 enzyme function.

Key words: mRNA expression, ERN1 knockdown, MAP4K3, RIPK1, hypoxia, U87 glioma cells



Similar Articles

Promoter CAG is more efficient than hepatocyte‑targeting TBG for transgene expression via rAAV8 in liver tissues.
Kang J, Hang L, Zheng W, Luo J, Zhang X, Song Y, Liu A
Molecular medicine reports. 2022; 25(1):

The promise of neuroprotection by dietary restriction in glaucoma.
Russo R, Nucci C, Adornetto A
Neural regeneration research. 2022; 17(1): 45-47

Biovalue in Human Brain Banking: Applications and Challenges for Research in Neurodegenerative Diseases.
Vedam-Mai V
Methods in molecular biology (Clifton, N.J.). 2022; 2389(): 209-220

Generating Monocyte-Derived Endothelial-like Cells for Vascular Regeneration.
Smith RJ, Andreadis ST
Methods in molecular biology (Clifton, N.J.). 2022; 2375(): 13-19

MTA1, a metastasis‑associated protein, in endothelial cells is an essential molecule for angiogenesis.
Ishikawa M, Osaki M, Uno N, Ohira T, Kugoh H, Okada F
Molecular medicine reports. 2022; 25(1):


Full-text options


Latest Statistics about COVID-19
• pubstat.org


Add your Article(s) to Indexes
• citeindex.org






Covid-19 Trends and Statistics
ScopeMed.com
CiteIndex.org
CancerLine
FoodsLine
PhytoMedline
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright © ScopeMed® Information Services.



ScopeMed Web Sites