Home|Journals|Articles by Year

Directory for Medical Articles
 

Original Article

J App Pharm Sci. 2014; 4(8): 011-017


Evaluation of in-vitro apoptosis induction, cytotoxic activity of Hymenodictyon excelsum (Roxb.) Wall in Dalton’s lymphoma ascites (DLA) and Lung fibroblast - Mouse L929 cell lines

Kalathil Khairunnisa, Dhananjayan Karthik.


Abstract

The purpose of present study was to investigate the methanolic bark extract of Hymenodictyon excelsum (Roxb) Wall for its apoptosis, cytotoxic activities. Induction of apoptosis was carried out on L-929 cell line. The cytotoxic activity was evaluated by MTT assay against lung fibroblast (L-929) cell line and trypan blue dye exclusion assay on dalton’s lymphoma ascites(DLA) cells. Morphological changes and DNA fragmentation were found upon incubation with extract. The extract was found to be cytotoxic towards L-929 cells in 72 h MTT assay and concentration required for 50% cell death was 3.85µg/ml. Thus from the present investigation it can be concluded that the methanolic bark extract of Hymenodictyon excelsum (Roxb)Wall exhibited apoptosis induction and cytotoxic activities.

Key words: Apoptosis, cytotoxicity, Hymenodictyon excelsum, MTT assay, Neoplasia.






Full-text options


Share this Article



Online Article Submission
• ejmanager.com







eJManager.com
Review(er)s Central
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.