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Research Article



Antibiotic susceptibility and biofilm formation among lactose fermenting Gram negative bacilli causing urinary tract infection

Reshma Kottila, Vimalin Hena.




Abstract

Background:
A urinary tract infection is an epidemic infection of any part of the urinary system, which is very common, causes serious health problems and morbidity. UTI mainly caused by bacteria, fungus, parasites, viruses. Both gram negative and gram positive bacteria were causes UTI, among this majority of pathogens are gram negative bacilli. Very common bacterial pathogens are E.coli, klebsiella, enterobacter, proteus and pseudomonas.
Aim:
The study helps to know the importance of lactose gram negative bacilli in urinary tract, status of drug susceptibility among those bacteria. Since biofilm formation leads to persistence of infection and drug resistance among bacteria, this study will provide information on status of biofilm formation among lactose fermenting gram negative bacilli causing urinary tract infection.
Methods:
The study was performed during the year MAY 2019 to AUGUST 2019 at the Department of Microbiology, Yenepoya Medical College Derlakatte, Mangalore, Karnataka. Almost 86 urine samples were collected during the study period. From the culture plate identified lactose fermenting and non-lactose fermenting organisms, my study was focused on lactose fermenting organisms.
Results:
During the study period 86 samples were collected and processed, from which E.coli (60.46%) is the most common pathogen that was isolated from the urine and second most bacteria is klebsiella(32.55%) followed by Enterobacter (6.99%). The study shows that E.coli mainly affects females than males.
Conclusion:
In this study I observed that females are the most important group which causes urinary tract infection very easily as opposed to males. The most habitual uropathogens was E.coli, Klebsiella, and Enterobacter. Those organisms show resistance to most of the antibiotics.

Key words: Gram negative bacilli, Biofilm, Urinary tract infection, Resistance towards antibiotics






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