Abstract

Extracts obtained by supercritical carbon dioxide extraction (SCE) and pressurized liquid extraction (PLE) from the Ajuga iva aerial parts growth in three different geographical sites of Alegria were analysed. Tocopherols in SCE Extracts were quantified by HPLC, ?-tocopherol dominated the profile in both A. iva of Bejaia (BEJ) and Bordj Bou Arreridj (BBA), while ?-tocopherol was the lowest. PLE method produced higher extraction yields than SCE method, which ranged from 0.31 to 24.95% (w/w), the water gave the best yields extraction. Total phenolic content (TPC) ranged from 15.60 to 63.45mg GAE/g DWE; also the water gave higher total yield of phenolics, and the best TPC from DWP. The antioxidant activity was assessed by different assays, water and SCE extracts showed a slightly higher antioxidative effect than that obtained by Ethanol and Acetone. A slight opposite trend was obtained with the FRAP antioxidant activity; Acetone was the best extract after SCE extracts. Small differences in antioxidant activity values were found between the different sites of A. iva: BEJ, BBA, and ELO.

Key words: Antioxidant activity, Ajuga iva, SCE, DPPH•, FRAP, H-ORAC, L-ORAC