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Open Vet J. 2023; 13(11): 1416-1424

Variations in metabolic parameters of in vitro matured porcine oocytes after vitrification-warming

Sergio Morado, Ailen Aparicio, Daniela Pinchetti, Claudia Cecilia Arraztoa, Gabriel Alvarez, Cynthia Gutnisky, Deborah Neild, Gabriel Dalvit, Pablo Cetica.

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As the porcine oocyte is the most sensitive to low temperature damage, it has been difficult to cryopreserve compared to those from other domestic animals. However, at present, vitrification is used as a method for the cryopreservation of both oocytes and embryos in this species.
Our aim was to analyze alterations in metabolic parameters in vitrified-warmed in vitro matured porcine oocytes at different post-warming recuperation times. Additionally, metaphase II plate recovery time analysis, in vitro fertilization and intracytoplasmic sperm injection were carried out to evaluate oocyte recovery capacity.
Oocytes were vitrified-warmed and then incubated for 0, 3 or 21h post-warming to assess biochemical parameters.
Oocyte viability and morphology were not affected by vitrification-warming. Cytosolic oxidative status, active mitochondria and reactive oxygen species levels presented changes at the different time points in control and vitrified-warmed oocytes (P

Key words: Cryopreservation, Mitochondria, Porcine oocytes, Reactive oxygen species, Vitrification

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