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Original Article

J App Pharm Sci. 2014; 4(11): 008-014

Improvement of cell mass production of Lactobacillus delbrueckii sp bulgaricus WICC-B-02: A newly isolated probiotic strain from mother’s milk

Elsayed A. Elsayed, Nor Zalina Othman, Roslinda Malek, Tebbie Tang, Hesham A. El Enshasy.


Lactobacillus delbrueckii sp bulgaricus WICC-B-02 is new probiotic strain which was initially isolated from the mother’s milk. As lactic acid bacterium, it’s known as a highly efficient probiotic microorganism with a wide range of benefits on the human health. This study was conducted to design and establish industrial platform for high cell mass production of L. delbrueckii sp bulgaricus for pharmaceutical and food industries application. However, due to low cell mass production caused by the accumulation of lactic acid during the cultivation of lactic acid bacteria, therefore, the optimization of cell mass production with low lactic acid production was developed in this study. The new formulation of the production medium was developed by optimizing the main components such as glucose (30 g.L-1), yeast extract (6.0 g.L-1) and peptone (6.0 g.L-1) in shake flask cultivation. The cell mass production was 3.14 g.L-1, and it increased to 6.08 g.L-1 with the lactic acid production being reduced by about 33%, compared to the un-optimized medium.

Key words: Probiotics, Process development, Mother’s milk, Lactobacillus.

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