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A study of aerobic and anaerobic bacteria in diabetic foot ulcer and in vitro sensitivity of antimicrobial agent

Aasha Halpati, Kairavi J Desai, Ravindra Jadeja, Mayur Parmar.

Background: Diabetes mellitus is a chronic disorder affecting large population and a major public health problem .India ranking first in the world for diabetes. Diabetic patients are vulnerable to foot ulcer with polymicrobial infection.

Aims & Objective: Study was aimed to know etiology as well as antibiotic sensitivity that helps clinicians to select appropriate antimicrobial therapy.

Materials and Methods: The study was done from July 2010 to August 2011, in department of Microbiology, Sir T hospital and government medical college, Bhavnagar. 120 patients included from surgery dept. and samples were collected for aerobic and anaerobic culture, further identification was done by microscopy, culture and biochemical reaction. The antibiotic sensitivity testing was performed by Kirby Bauer method of disk diffusion using NCCLS guidelines.

Results: Out of 120 cases, total 150 bacterial species isolated with maximum aerobes (84%) and Staphylococcus aureus and Pseudomonas aeruginosa were commonest aerobic bacteria responsible for infection. Out of 24 Anaerobic isolates, 62.5% was Bacteroides species. Gram positive bacteria were sensitive to Vancomycin (100%) and gram negative bacteria were sensitive to Imipenam, Meropenam and Levofloxacin.

Conclusion: The study concludes that diabetic foot ulcer infection is always polymicrobial in nature. There was resistant pattern of aerobic bacteria to cephalosporin group may be due to indiscriminate usage of antibiotics. So, it is mandatory to screen all elder patients for diabetes as well as risk factors. Periodic surveillance is necessary to know multidrug resistant strains of hospital and to apply proper antibiotic policy.

Key words: National Committee for Clinical Laboratory Standards (NCCLS) Guidelines; Diabetic Foot Ulcer; Bacteria

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