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In Vivo Alteration of Phase II Transformation Enzyme (GST) Levels by Stainless Steel Orthodontic Appliances

Dilip Daniel Quadras,Krishna Nayak U S,Suchetha Kumari N,Vijay R, Priya Gatty,Prashant Pujari.

Abstract
Orthodontic appliances includes bands, brackets and wires which are made up of stainless steel with the composition of roughly 8-12% nickel and 17-22% chromium and other trace metals. Corrosion of orthodontic appliances can have a toxic effect on surrounding oral tissues. The main aim of the study is to analyze the Glutathione s transferase activity in saliva and blood samples of patients undergoing Orthodontic treatment. The study group included 40 participants and Samples were taken in different time intervals: Group 1- Collection of samples before the appliances fixed, Group 2- Collection of sample after one week of fixed appliances, Group 3- Collection of sample after three months of fixed appliances each from the Orthodontic Department of A.B Shetty Memorial institute of dental sciences. Glutathione S transferase was estimated using 1-chloro, 2, 4-dinitrobenzene (CDNB) method. In case of Saliva group 3 (211.17±1.84) significantly increased compare to group 1 (87.05±3.86) and group 2 (118.74±2.8). In case of RBC lysate group 2(532.33±3.2) and group 3(512.40±1.16) were increased when compare to group 1(107.12±2.54). The Glutathione s transferase levels of patients increased over a period of time after treatment with orthodontic appliances but other factors like age of the patient, diet rich in antioxidants, light and strenuous physical activity, medication, amalgam fillings and genetic constitution of an individual also influenced the results.

Key words: Stainless steel orthodontic appliances, Glutathione S transferase, in vivo study


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