The infection of Trypanosoma evansi was screened in 400 blood samples of buffaloes belonging to thirty villages in seven blocks of district Hisar, Haryana using Giemsa staining of thin blood smear and monoclonal antibody based latex agglutination test (mAb-LAT). The haemoglobin (g/dl), packed cell volume (%) and the body temperature (oF) were also examined. The blood examination did not revealed any positive for Trypanosoma evansi. However, mAb-LAT showed 95 (23.7%), 101 (25.2%), 84 (21%), 38 (9.5%) and 82 (20.5%) sample as strong, moderate, weak positive, doubtful and negative, respectively. Significant difference were observed in haemoglobin and PCV values between positive results in comparison to suspected and negative results of mAb-LAT. The body temperature also showed significant difference between positive results when compared to negative results of mAb-LAT. The study signify the poor efficacy of blood examination and recommends the use of immunological tests for the diagnosis of trypanosomosis.
Key words: Trypanosoma evansi, Buffaloes, mAb-LAT, Haryana