Lidocaine (LDC) and Prilocaine (PLC) is estimated in a topical local anaesthetic cream using a direct, eco-friendly, stability indicating gas chromatographic technique with flame ionization detector (FID). The mixture of LDC and PLC was separated using Zebron DB drug column. The column temperature and flow rate was 230ᴼ C and 14 mL/minutes respectively. The retention time was found to be 5.1 minutes for PLC and 5.4 minutes for LDC. Linearity was observed in the concentration range of 20-100 μg/mL and 10-50 μg/mL for LDC and PLC respectively. The method was validated according to International Conference on Harmonization guideline and values of linearity, limit of detection, limit of quanfication, precision, accuracy were found to be in good accordance with the prescribed value. A direct, stability-indicating method was developed for the determination of Lidocaine and Prilocaine in topical dosage forms in presence of its degradation products. The proposed method can be useful in the quality control of Lidocaine and Prilocaine in their topical formulation.
Key words: Gas chromatography, Green Chemistry, ICH guidelines, Lidocaine, Prilocaine, Simultaneous estimation
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