Home|Journals|Articles by Year|Audio Abstracts

Original Research

Molecular characterization of enterotoxin and antibiotic resistance genes-producing Staphylococcus aureus derived from imported meat: Artemisia herb‐alba extract as an antibacterial agent

Shimaa El-Sapagh,Mohamed O. Abdel-Monem,Esraa Badr,Mohamed H. Yassine,Nesma Elsayed,Awad Y. Shala.


Staphylococcus aureus is a major foodborne pathogen in raw and ready-to-eat meat products. In this study, the effectiveness of methanolic extracts of Artemisia herbal-alba in inhibiting pan-resistant and enterotoxic Staphylococcus aureus was investigated. The first analysis showed a high prevalence (33%) of coagulase-positive staphylococci in imported meat samples from retail stores. Examination of antibiotic resistance patterns revealed that staphylococcal antibiogram resistance profiles were diverse: five strains demonstrated resistance to at least one antimicrobial agent, fourteen isolates demonstrated multidrug resistance (MDR) and one resistant to all test antibiotics (PAN). Multiplex PCR of pan-Staphylococcus strains revealed positive serotypes for the enterotoxin genes seb, seg and sei at 665.05 bp, 277.99 bp and 460.74 bp, respectively, and a positive serotype for the mec-A gene at 538.45 bp. Compared to the tetracycline effect, methanolic extracts of Artemisia herba-alba showed significant inhibitory effects against pan-resistant Staphylococcus aureus. Real-time quantitative PCR data analysis showed that Artemisia herba-alba methanolic extracts caused a significant decrease in the expression levels of the genes mecA, mecC, sei, seg and seb. Therefore, the methanolic extract of Artemisia herba-alba has the potential to be a promising natural product to control foodborne pathogens and could be valuable for food safety applications.

Key words: Staphylococcal food poisoning, antimicrobial activity, Artemisia herba-alba, staphylococcal enterotoxins, MDR, Extracts

Full-text options

Share this Article

Online Article Submission
• ejmanager.com

ejPort - eJManager.com
Refer & Earn
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under Creative Commons Attribution 4.0 International License (CC BY), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.