The study was conducted to investigate the effect of enrofloxacin administration on aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (AKP) and acid phosphatase (ACP) in seminal plasma of Barbari bucks. The bucks were administered enrofloxacin at the dose of 5mg/kg body weight intra-muscularly daily for 7 days. From each buck biweekly eighteen ejaculates (1st to 18th) were collected and one sample (0th) from each buck was collected before administration of enrofloxacin buck using a non-oestrous doe as a dummy. Seminal plasma was obtained after centrifuging semen and AST, ALT, ACP and AKP level was estimated besides protein concentration. AST and ALT levels were significantly higher (P≤0.01) and maximum in seminal plasma of 9th and 5th ejaculate respectively, as compared to ejaculate at 0 day. The AKP and ACP level increased significantly (P≤0.01) and was the maximum in the seminal plasma of 1st and 4th ejaculate respectively, as compared to ejaculate at 0 day. It may be concluded from this study that only after nine weeks of enrofolaxacin administration, the biochemical status of seminal plasma with respect to ALT, AST, AKP and ACP becomes normal which may be attributed to residual effect of enrofloxacin.
Key words: Enrofloxacin, Seminal Plasma, Biochemistry