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Influence of low temperature storage on the properties of human placenta extracts

Oleg A. Nardid, Denys N. Pogozhykh, Svetlana V. Repina, Svitlana L. Rozanova, Ievgeniia I. Naumenko, Ekaterina D. Rozanova.


Abstract
Cited by (1)

Objective: This research represents the results of comparative investigations of the composition and biological activity of aqueous-saline extracts, obtained from fresh and stored at –196ºC human placental tissues.
Methods: The studying of quantitative composition of proteins in human placenta extract (HPE) was performed with gel-chromatography method. Content of lipid peroxidation products and catalase activity in the extracts were evaluated using spectrophotometric assay. Biological activity of extracts was evaluated by studying the modifications of structural and functional parameters of erythrocytes after exposure with the extracts.
Results: Our researches have shown that properties of HPE from the stored at –196ºС tissues slightly differ from those of fresh tissues. In extract of frozen tissues the content of high-molecular proteins is relatively higher. Content of malonyldialdehyde in extracts of the tissues stored at –196ºC does not change. Catalase activity decreases. For evaluation of biological effect of the extract on erythrocytes we have analyzed the following structural and functional parameters of red blood cells: spontaneous hemolysis, osmotic, and low-pH resistances, which characterize mechanical properties and stability of cellular membranes; relative content of main forms of hemoglobin (oxy-, deoxy-, and met-Hb), which elucidate the functioning of biochemical systems; and cytosol microviscosity, which reflects structure-dynamical state, determined, first of all, by interaction of cytosol and components of cytoskeleton with membranes.
Conclusion: It was shown that distinctions between the effects of extracts on erythrocytes were mainly manifested at cellular membrane level, not affecting main vital parameters.

Key words: Biological activity; Erythrocytes; Low-temperature storage; Placental extract






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