First report of Aliarcobacter cryaerophilus in ready-to-cook chicken meat samples from super shops in Bangladesh
Md. Muket Mahmud, Ajran Kabir, Md. Zawad Hossain, Sanjida Jamal Mim, Israt Jahan Yeva, Minara Khatun, Mohammad Saidur Rahman, Madan Mohan Dey, K. H. M. Nazmul Hussain Nazir.
Objective: This study aimed to isolate Aliarcobacter cryaerophilus in ready-to-cook poultry meat in Bangladesh.
Materials and Methods: Thirty drumstick samples were collected from super shops in Dhaka city (n = 10), Mymensingh city (n = 10), and Patuakhali town (n = 10). After sample processing, they were cultured in Blood agar media with Campylobacter base using a microfilter (0.42 nm). Suspected colonies were subjected to DNA extraction and PCR assay targeting 16SrRNA genes. Then, sequencing was performed for confirmation.
Results: Of 30 samples, 3 (10%) were positive for A. cryaerophilus. Phylogenetic analysis shows that our isolate has strong similarities with one of the isolates from China.
Conclusion: The presence of this organism in ready-to-cook poultry meat is a significant concern for consumers as it bears zoonotic importance.
Key words: Aliarcobacter cryaerophilus; 16SrRNA; PCR; phylogenetic analysis; poultry meat; super shops