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Research Article

Mouthwash Preparation Using Stevia and Neem Based Silver Nanoparticles and Its Cytotoxic Activity

Nandini Palanivel, R Priyadharshini*, S. Rajeshkumar, Palati Sinduja.


Introduction: Azadirachta indica (Neem) may be a rapid-growing plant even in severe drought which shed most of its
leaves. The branches are wide and spreading. In this present investigation, we have prepared mouthwash using Stevia and
Neem-based silver nanoparticles.
Materials And Methods: Azadirachta indica and Stevia rebaudiana plant powder form was taken. 1g of Neem and Stevia
extract powder mixed with 100ml of distilled water and then it was boiled, cooled and filtered with filtered paper and
allowed to settle and the extract mixture was found to be 50ml and filtered.
Results And Discussion :The plant had exhibited a potent cytotoxic effect at higher concentrations. Extracellular synthesis
of silver nanoparticles using Neem and Stevia was primarily identified by visual identification; the appearance of silver
and whitish colour in reaction mixture indicates nanoparticles.
Conclusion :The present study revealed that the silver nanoparticles can be synthesised in a simple eco-friendly method
using Neem and Stevia .

Key words: Azadirachta indica, Stevia rebaudiana , Silver nanoparticles,Cytotoxicity, Green synthesis.

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