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Research Article

Comparative Effect of Various Irrigants with Herbal Formulations in Reducing Enterococcus Faecalis Count

J Deepasakthi, R.V.Geetha*, T. Lakshmi.


Background: Enterococcus faecalis is associated with different forms of periradicular diseases, both primary and
persistence endodontic infections. Traditional herbals have high antimicrobial, anti-inflammatory, antioxidant and
biocompatible properties that make their use in dentistry more extensive. Judicious use of these herbal formulations has
been found to mitigate the E.faecalis count.
Aim: To compare the effect of various irrigants with herbal formulations in reducing Enterococcus faecalis count
Materials and methods: Different herbal formulated irrigants were procured commercially. The test organism E.faecalis
was isolated and maintained in the Nutrient Agar Slope at 4 degree C. Different concentrations of herbal irrigants were
mixed with BHI agar and 50μL of the test organisms were added and poured into sterile petri dishes. Lawn culture of the
test bacteria was made on the Plates and incubated at 37 degree C for 24 hrs and the colony forming units(CFU) were
counted and recorded.
Result: In this present study, comparing all 3 samples there is a higher reduction of count in Sample 3 (Cardamom and
Tulsi mediated) than Sample 1 and Sample 2.
Conclusion: Based on the results presented, it is possible to conclude that the herbal formulations represent a good
reduction of Enterococcus faecalis.

Key words: Enterococcus faecalis,Nutrient Agar Slope,HerbalIrrigants,BHIagar

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