Home|Journals|Articles by Year|Audio Abstracts

Original Article

Med Arch. 2021; 75(6): 436-443

Expression of Inflammatory Mediators in Periodontitis Over Established Diabetes: an Experimental Study in Rats

Charbel Choubaya, Nathalie Chahine, Georges Aoun, Sukumaran Anil, Pierre Zalloua, Ziad Salameh.


Background: Diabetes mellitus is characterized by hyperglycemia ensuing from deficiencies in insulin action, secretion, or both. Hyperglycemia has wide-ranging molecular and cellular effects, leading to oxidative stress, up-regulation of pro-inflammatory responses, and vascular changes. Objectives: The aim of this study was to evaluate the expressions of inflammatory markers involved in periodontal destructive process occurring in diabetes, periodontitis (PD), and both coexisting conditions. Methods: A rat model was carried out using streptozotocin (STZ) to induce diabetes and Lipopolysaccharides (LPS) with teeth ligature to mimic periodontitis. The animals were distributed randomly into seven groups (n=12) and treated for 10 weeks with alternation between diabetes and PD. The relative quantification analysis of inflammatory markers expression: CRP, MMP-2-14, TIMP-2, IL-4, IFN-γ, was performed at the end of the experiments using western blot after protein isolation from periodontal tissue surrounding the ligation. Results: The data showed that CRP, MMP-2, MMP-14, TIMP-2, and IFN-γ are involved in the process of periodontal inflammation associated with diabetes. A significant increase (p

Key words: Diabetes, inflammatory markers, periodontitis, periodontal inflammation, streptozotocin.

Full-text options

Share this Article

Online Article Submission
• ejmanager.com

ejPort - eJManager.com
Refer & Earn
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under Creative Commons Attribution 4.0 International License (CC BY), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.