A subchronic toxicity test of the n-hexane and ethyl acetate fraction combination of rose myrtle leaves on male white rats was performed to determine its safety after repeated administration. There were four groups, each consisting of five rats, with two rats as a satellite of each group. Group I acted as a control group which was only given 0.5% Na CMC. Groups II, III, and IV were the test groups for the combination of rose myrtle leaf fraction at a dose of 200, 400, and 800 mg/KgBW. The results showed no toxic symptoms, the change in weight of rats for 28 days and 42 days in each group was not significantly different (P > 0.05). The administration of the rose myrtle leaf fraction combination did not affect the levels of Hb, erythrocytes, and leukocytes, as well as SGPT, creatinine, and urea on rats significantly (P > 0.05). At a dose of 800 mg/KgBW, the fraction combination significantly (P < 0.05) affected the levels of SGOT in rats. The average SGOT levels on day 29 in the control group and the group with a dose of 200, 400, and 800 mg/ KgBW, respectively, were 257.63, 224.80, 251.19, and 306.92 IU/L. The organ macroscopy of the liver, kidney, and heart in each group indicates no significant difference (P > 0.05). The combination of rose myrtle leaf fraction did not cause damage to the liver, kidney, and heart. The n-hexane and ethylacetate fractions from karamunting leaves are potential to be used as raw materials for diarrhea medicine for shigellosis and salmonellosis infections.
Key words: Rhodomyrtus tomentosa, 28-days subchronic toxicity, hematology, biochemistry of blood, and histology
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