Introduction: In recent studies copper nanoparticles have drawn attention due to its extensive properties in medicine for curing various diseases and stabilising our immunity. Blue tea, which comes from the Clitoria ternatea plant, also known as the butterfly-blue pea, blue-pea, or Asian pigeonwings, has been around for centuries. It's become increasingly common as a result of its numerous health benefits. This herbal blue tea is really a tisane, which means it's caffeine-free.
Aim: The aim of the study was to evaluate the anti-inflammatory and antioxidant properties of copper nanoparticles synthesised using blue tea.
Materials and Methods: Preparation of the blue tea extract, synthesis of copper nanoparticles, preparation of nanoparticles powder and then antioxidant and anti-inflammatory activity of nanoparticles were analysed. Antioxidant activity and anti- inflammatory activity was assessed using DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) assay and inhibition of albumin denaturation assay. The standard used was diclofenac sodium in various concentrations.
Results: The formation of copper nanoparticles was indicated by the peak found in the spectroscopy. Copper nanoparticles synthesized using blue tea showed highest absorbance at a concentration of 50μl (85.2%) when it was subjected to DPPH assay to check for its antioxidant property at a wavelength of about 517 nm. Copper nanoparticles synthesized using blue tea showed highest absorbance at a concentration of 50μl (91.4%) when it was subjected to inhibition of albumin denaturation assay to analyse for its anti-inflammatory activity at a wavelength of about 660 nm.
Conclusion: The present study suggests that blue tea-mediated copper nanoparticles have good antioxidant and anti-inflammatory properties. It may be concluded that antioxidant and anti-inflammatory activity of blue tea is not as effective as Diclofenac, but it can be more effective when its concentration is increased to acceptable levels.
Key words: Antioxidant, Anti inflammatory, Clitoria ternatea, Copper nanoparticles, Green synthesis, Innovative.
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