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Preparation of Mouthwash Using Red Sandal Mediated Silver Nanoparticles and Its Antimicrobial Activity - An In Vitro Study



Introduction: The Indian native herb Pterocarpus santalinus L. is being used to manage a variety of ailments.The potent properties of various phytochemicals found in P. santalinus L. and its antimicrobial activity interested us to analyse their efficiency. The aim of the study is to prepare mouthwash using red sandal mediated silver nanoparticles and evaluate its antimicrobial activity
Materials and Methods: Silver nanoparticle is synthesized by using Pterocarpus santalinus L., followed by characterization of nanoparticles with UV-VIS spectrophotometer, Mouthwash was prepared from its and tested for antimicrobial activity of using agar well diffusion method.
Results: Mouthwash had a better effect on S.Aureus, with a maximum inhibition zone of 23mm at 100μL and on C.Albicans, at 100μL, it had a 27mm zone of inhibition. Thus as the concentration increases the zone of inhibition also increases.
Conclusion: In conclusion, we have demonstrated that 0.1% mouthwash prepared from Pterocarpus santalinus mediated silver nanoparticles exhibit excellent dose dependent inhibition activity against the pathogens Streptococcus mutans, Staphylococcus aureus, Enterococcus faecalis and Candida albicans. Hence, red sandal mediated silver nanoparticles proves to be a safer alternative for infections caused by oral pathogens Streptococcus mutans, and fungal species like Candida albicans.

Key words: Antimicrobial Activity, Agnps ,Pterocarpus Santalinus, Herbal Mouthwash, Oral Pathogens, Green Synthesis.

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