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Antimicrobial Activity of Green tea and Mint Extract against Wound Pathogens

SAISHREE ANCHANA RAJESWARAN, LAKSHMINARAYANAN ARIVARASU, RAJESHKUMAR S, LAKSHMI THANGAVELU.


Abstract

Introduction: Wound healing may also be affected by the growth of certain wound pathogens including Pseudomonas, Escherichia coli, Staphylococcus aureus. Camellia sinensis, or the Green tea, has large quantities of catechins and their derivatives called polyphenols which are known for their antioxidant, anti-cancer effects. Mint or Mentha is a plant, also having established antimicrobial, antioxidant, anticancer, antiemetic, and blood purification properties.
Aim: To establish the antimicrobial properties of Green Tea and Mint extract on wound pathogens
Materials and Methods: The plant extract was prepared with 1g dried green tea leaves and 1g dried mint leaves boiled for 10 minutes in 100ml distilled water, at 60-80°C on a heating mantle. The extract was filtered using Whatman No.1 filter paper. Three Mueller-Hinton agar plates with microbial suspensions of Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus dispensed on each of their four wells, were prepared. Three of the four wells in a plate were suspended with 25 μL, 50 μL, 100 μL of the prepared extract, with the fourth as a standard. The prepared plates were then incubated for 24 hours at 37°C, and the zone of inhibition for each sample was recorded.
Results: At 100 μL of the extract for all the three organisms, Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus, 25mm, 28mm and 27mm were the zones of inhibition obtained, which were the maximum recorded.
Conclusion: In the present study, it is observed that the higher the concentration of the extract, greater is the zone of inhibition for the extract against the organism tested.

Key words: Camellia sinensis, Mentha, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, antimicrobial activity, wound pathogens






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