Home|Journals|Articles by Year|Audio Abstracts
 

Original Article



Application of the central composite design approach for optimization of the nanosilver formula using a natural bioreductor from Camellia sinensis L. extract

Rini Dwiastuti, Phingkan Alamanda Suhendra, Sri Hartati Yuliani, Florentinus Dika Octa Riswanto.




Abstract
Cited by 0 Articles

In recent years, the interest in applying the nanosilver technology in the medical field has increased due to its benefit for microbial inactivation. A natural bioreductor was chosen and developed in the nanosilver formulation to minimize the toxicity effects. The content of rutin makes it possible to develop an alternative bioreductor agent using black tea (Camellia sinensis L.) leaves extract. The aim of this research was to optimize the nanosilver formula consisting of black tea leaf extract and AgNO3 with the employment of central composite design. The visible absorption wavelength and transmittance percentage were observed as dependent variables. The presence of rutin in the black tea leaves extract was proved using the thin-layer chromatography (TLC) technique. It was found that the extract concentration of 2.131% (m/v) and the AgNO3 concentration of 1.379 mM were stated as the computational recommendation resulting from the predictive model with a composite desirability value at 0.998. These optimum conditions were applied in the synthesis of six nanosilver formula replications and resulted in the percentage of a prediction relative error of absorption wavelength and transmittance which were in the ranges of 1.18%–9.18% and 2.72%–8.64%, respectively. The Relative Standard Deviation (RSD) values of absorption wavelength and transmittance were 2.81% and 2.21%, respectively. The Z-average of the nanosilver particles was 124.8 nm.

Key words: bioreductor, Camellia sinensis L., optimization, response surface methodology






Full-text options


Share this Article


Online Article Submission
• ejmanager.com




ejPort - eJManager.com
Refer & Earn
JournalList
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under Creative Commons Attribution 4.0 International License (CC BY), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.