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Optimization of cultural conditions of gellan gum production from recombinant Sphingomonas paucimobilis ATCC 31461 and its characterization

Soumiya Sukumar, Santhiagu Arockiasamy, Manjusha Chemmattu Moothona.




Abstract
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Microorganisms that produce exopolysaccharides have currently gained broad attention and are a subject of great concern for modern-day biotechnologists and microbiologists. Sphingomonas paucimobilis is a Gram-negative rod-shaped bacterium isolated from the Elodea plant tissue. The effect of the culture conditions on gellan gum production by recombinant S. paucimobilis ATCC 31461 in a stirred-type bioreactor was investigated. The products of fermentation were characterized by Fourier transform infrared (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC). The FTIR spectrum revealed characteristic peaks of different functional groups in the gellan. SEM was carried out to observe the morphology of the gellan gum, while the thermal stability of the biopolymer was determined by TGA and DSC analysis. The compositional analysis of the purified gellan showed that it is a heteropolymer containing glucose, rhamnose, and glucuronic acid. The compression test of the gellan revealed its textural property. Maximum gellan gum was attained after 48 h of incubation at 30°C with a pH of 6.5, 500 rpm, and 100% dissolved oxygen. The rheology study indicated the non-newtonian nature of gellan gum. The solubility of gellan gum in different polar and non-polar solvents was also investigated. The findings in this study showed that gellan gum produced by recombinant S. paucimobilis ATCC 31461 has a high potential to be utilized in the pharmaceutical and food industry.

Key words: Compression, Exopolysaccharide, Recombinant, Rheology, Thermogravimetric analysis






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