Angiopteris ferox Copel is one of the plants empirically used as an anticancer by the Dayak tribe, native people groups in Indonesian Borneo, most of whom traditionally lived with maritime culture in West Kutai, Indonesia. The purpose of this investigation was to determine antioxidant and cytotoxic effects from extract and fractions of A. ferox Copel and to evaluate their chemical contents using the liquid chromatography-mass spectrometry (LC-MS) approach. A. ferox Copel was extracted with ethanol and fractionation was done using various solvents. Extract and fractions were evaluated to their antioxidant activity and cytotoxic effect against three types of cancer cell lines, that is, epithelioid carcinoma, human colon cancer, and mammary gland breast cancer, and their chemical compounds by LC-MS. The study exhibited that the ethyl acetate fraction has contributed to antioxidant with very strong activity and cytotoxic with moderate activity against the three types of the cancer cell lines. LC-MS prediction shows that phenolic and flavonoid groups compounds the response to their effect. Therefore, A. ferox Copel is very prospective to be developed as anticancer although its sample still needs further testing.
Key words: A.ferox Copel, Antioxidant, Cancer Cells, Cytotoxic, LC-MS.