context: pleurotus species (oyster mushroom) have been used by people worldwide for their medicinal properties, dietary
benefit and other purposes. They contain various compounds which biologically active with therapeutic effects. Pleurotus florida
which also known as white oyster mushroom is an edible mushroom that is popular due to its low production cost, nutritional
contents and easy cultivation method. Aims: this study was designed to evaluate the potential antioxidant and antimicrobial
properties of pleurotus florida exopolysaccharide and endopolysaccharide extracts to be an alternative source for existing
antioxidant and antimicrobial agent.
Methods: In this study, the antioxidant activities of different concentrations of Pleurotus florida extracts were evaluated using total
phenolic content (TPC), DPPH radical scavenging activity and ferric reducing antioxidant power (FRAP) assay and the
antimicrobial activities were evaluated using agar disk-diffusion method.
Results: Out of the 5 concentrations tested for antioxidant properties for both extracts, concentration of 1mg/ml of the IPS extract
showed the highest TPC and FRAP activity, while for the DPPH radical scavenging activity concentration of 0.2mg/ml of IPS
extract showed the highest scavenging activity. For the antimicrobial evaluation, all the 3 concentrations that were used showed no
antimicrobial activity against E. coli, S.aureus and P. aeruginosa.
Conclusion: In conclusion, white oyster mushroom has shown potent antioxidant effects which makes it a good antioxidant
candidate that can be incorporated in medicine and diet, and as the concentration of Pleurotus florida mushroom increases the
antioxidant effects increase. However, both exo-and endopolysaccharide extract doesn’t exhibit any antimicrobial activity in low
Key words: Antioxidant; Antimicrobial; Endopolysaccharide; Exopolysaccharide; Mushrooms; Pleurotus florida