All parts of Holoptelea integrifolia, a roadside plant, are suggested by Charaka Samhitha
Sushrutha Samhita and other traditional systems for the treatment of inflammation, acid
gastritis, dyspepsia, flatulence, colic, intestinal worms,vomiting, wounds, vitiligo, leprosy,
filariasis, diabetes, haemorrhoids, dysmenorrhoea, and rheumatism.The present studywas
aimed at macroscopic, microscopic, and chromatographic evaluation of
Holopteleaintegrifolia leaves. The macroscopic,microscopic evaluation is carried out in terms
of organoleptic, microscopic, and physical parameters like fluorescence analysis, treatment of
plant drugs with different chemical reagents. The dried leaves were subjected to successive
Soxhlet extraction by taking the increasing order of solvents like petroleum ether,
chloroform, Acetone ,Benzene ethyl ,Water and methanol. These solvent extracts were
subjected to a phytochemical evaluation to detect the different chemical principles present i.e.
Protein, amino acids ,carbohydrates, steroids, alkaloids, glycosides, phenolic compound and
tannis. And its find out a chromatographic evaluation to isolatethe chemical compounds.
Macroscopic, microscopic, and physicochemical determination and chromatography
screening canbe used as a diagnostic tool in the correct identification of plants. By the above
studies, any adulterants present in these plants can be identified easily.The comparative and
multidisciplinary approach to the study of HolopteleaintegrifoliaRoxb Roxb
Key words: Organoleptic, Fluorescence, Microscopic, Chromatography, Photochemistry
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